动物造模,2型糖尿病 z-bio 2022-06-24 421

　　Objective: To observe the damage effect of busulfan on the function of hematopoietic system in KK/upj-Ay/J mice of type 2 diabetes mellitus and compare it with the control C57BL/6J(B6) mice.

　　Methods: Male KKAy mice were randomly divided into busulfan administration group and solvent control group. The former was injected with busulfan intraperitoneally at a dose of 40 mg/kg, and the latter was injected with the same dose of 5% DMSO. B6 mice were treated in the same way. grouping. 15d after administration, the mice were weighed, the immune organ coefficient was calculated, the peripheral blood count and classification of the mice were detected, and the proportion of hematopoietic progenitor cells, hematopoietic stem cells and long-term hematopoietic stem cells in the bone marrow was detected by flow cytometry. The function of mouse hematopoietic progenitor cells was assessed by a granulocyte-macrophage colony-forming unit assay.

　　Results: 15 days after busulfan administration, the body weight, white blood cells, red blood cells, hemoglobin, platelets, lymphocytes, neutrophils, HPC, HSC and LT-HSC ratios in the bone marrow of KKAy mice were significantly decreased, and CFU-GM The number was significantly decreased, and the proportion of spleen and thymocytes did not change significantly. After busulfan administration, the WBC, RBC, HGB and LYM of KKAy mice were significantly lower than those of B6 mice, but the ratio of PLT and HSC was significantly higher than that of B6 mice. The reduction of PLT and HSC in KKAy mice was significantly lower than that in B6 mice, while the reduction in HPC was significantly higher than that in B6 mice.

　　Conclusion: 40mg/kg busulfan administration can damage the hematopoietic system function of mice. After busulfan administration, WBC, RBC, HGB, and LYM were more severely damaged in KKAy mice, but PLT and HSC were less damaged and more tolerant, while hematopoietic progenitor cells were less tolerant than B6 mice.