动物造模,动脉粥样硬化 z-bio 2022-06-24 364

　　Objective: To study the effect and molecular mechanism of perilla seed oily extract (PSO) on reducing plaque formation and cardioprotection in ApoE-/- mice.

　　Methods: 3-month-old ApoE-/- mice were randomly divided into ApoE-/- group (no drug treatment), PSO low-dose group, PSO high-dose group and atorvastatin group, B6 mice were the control (WT group) , given a regular diet. After 8 weeks of continuous treatment, biochemical detection, HE staining, pathological detection, ultrasound detection, qPCR detection, ATP detection, and Pro-Q Diamond staining were performed.

　　RESULTS: Compared with the WT group, ApoE-/- mice had abnormal cardiac structure and function. After PSO treatment, the plaque area was reduced, and the cardiac structure and function were improved; the mRNA expressions of ANP, BNP, and β-MHC were down-regulated, the mRNA expressions of PPARα and PGC-1α were up-regulated, the intracellular ATP content was increased, and the phosphorylation level of TnI was increased.

　　CONCLUSION: PSO inhibits arterial plaque formation in ApoE-/- mice and maintains normal cardiac structure and function, which may increase ATP and myofilament phosphorylation, improve energy metabolism, and function by regulating the PPARα/PCG-1α/NRF-1 axis. Cardioprotective effect.