动物造模,糖尿病肾病 z-bio 2022-06-27 326

　　OBJECTIVE: To study the effect of quinsulfacyclohexanone on diabetic nephropathy in rats and its effects on the expression of angiotensin Ⅱ and vascular endothelial growth factor in the kidneys.

　　Methods: Forty 8-week-old SD healthy rats were selected, 10 of them were randomly selected as the normal group, and the remaining 30 rats were established as diabetic nephropathy rat models. After the model was successful, the two groups of diabetic nephropathy rats were randomly divided into model group of 10 and There were 20 rats in the treatment group, each 5 rats in the treatment group were injected with the same dose, and 5, 10, 15, and 30 mg/kg of quinsulfocyclohexanone were administered by gavage, respectively. The rats in the control group and the model group were given equal doses of physiological Saline was administered by gavage. The urine albumin excretion rate (Uaer) and glomerular filtration rate (GFR) of the rats in the administration group and the normal group were detected. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), insulin in the three groups Resistance index (HOMA-IR), 24h urinary microalbumin (24hU-mAlb), β2 microglobulin (β2-MG), blood urea nitrogen (BUN) levels were detected, and the chemotaxis of monocytes in rat kidney tissue was detected. Protein-1 (MCP-1) mRNA, lectin-like oxidized low-density lipoprotein receptor (LOX-1) mRNA and expression levels of Ang II and VEGF were compared between groups. Results When the dose was 15 mg/kg, rats were Uaer and GFR levels tend to be close to normal Uaer and GFR levels, so 15 mg/kg is considered to be an appropriate dose. The levels of IR, 24hU-mAlb, β2-MG, and BUN were higher than those in the normal group (P<0.05); the expression levels of MCP-1 mRNA, LOX-1 mRNA, AngⅡ, and VEGF in the kidney tissue of the rats in the model group and administration group The levels of FBG, HbA1c, HOMA-IR, 24hU-mAlb, β2-MG and BUN in the treatment group were lower than those in the model group (P<0.05). The expression levels of MCP-1 mRNA, LOX-1 mRNA, Ang II, and VEGF in kidney tissue were lower than those in the model group, with statistical differences (P<0.05).

　　CONCLUSION: Quinsulfocyclohexanone may improve the renal function and renal disease by inhibiting the expression of Ang II and VEGF in the renal tissue of diabetic nephropathy rats, and finally has the effect of renal protection.