动物造模,人脐带间充质干细胞 z-bio 2022-06-27 423

　　Objective: To investigate the mechanism of transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) to restore ovarian function in rats with premature ovarian failure.

　　Methods: 10 SPF SD female rats were randomly selected as the control group, and the other 20 rats were used to establish the POF model. The model rats were divided into a model group (10 rats) and a hUCMSCs transplantation group (10 rats). The changes of follicles and ovarian tissue ultrastructure of the rats in the group were compared, the levels of E2, FSH, AMH, ROS and 8-OHdG in serum were detected by ELISA, and the protein changes of SOD1 and UCP2 were detected by immunohistochemistry and Western blot.

　　Results: HE staining showed that compared with the control group, the number of atretic follicles in the model group increased, while the number of growing follicles decreased (both P<0.05). 0.05); ovarian electron microscope observation showed: after hUMSCs transplantation, rat ovarian cells appeared nucleus, nuclear membrane gradually recovered, and a few organelles appeared; ELISA results showed: serum LH, FSH levels, ROS and 8-OHdG production in hUCMSCs transplantation group Compared with the model group, the level of E2 in the hUCMSCs transplantation group was significantly decreased (P<0.05). Compared with the control group and the model group, the level of E2 in the hUCMSCs transplantation group was significantly increased (P<0.05). The number of UCP-2 and SOD1 positive cells increased, while the number of UCP-2 and SOD1 positive cells in the hUCMSCs transplantation group was lower than that in the model group. After hUCMSCs transplantation, the protein levels of SOD1 and UCP-2 were significantly lower than those in the model group (P<0.05).

　　Conclusion: hUMSCs transplantation can reduce the oxidative stress by reducing the expression of SOD1 and UCP-2, and finally achieve the effect of repairing ovarian function.