动物造模 z-bio 2022-06-27 296

　　OBJECTIVE: To observe the effect of a compound Chinese medicine prescription on lung injury in mice infected with influenza virus A/PR/8/H1N1.

　　METHODS: Ninety female BALB/c mice were randomly divided into 6 groups including blank control group, virus control group, amantadine hydrochloride group and compound traditional Chinese medicine high, medium and low dose groups, with 15 mice in each group. After 2 days of stomach infection, mice were intranasally infected to establish an influenza virus mouse model, and the blank control group was intranasally instilled with normal saline. 4 hours after virus infection, each group was given different doses of drugs or normal saline by intragastric administration, and then continued by intragastric administration for 5 days. On the fifth day of infection , Calculate the lung index of BALB/c mice in each group, determine the hemagglutination titer of lung tissue homogenate, and observe the pathological morphology of lung tissue.

　　RESULTS: Compared with the virus control group, the lung index of the amantadine hydrochloride group and the middle-dose compound Chinese medicine preparation group decreased significantly (P<0.01), and the lung index of the compound traditional Chinese medicine preparation low-dose group and the compound traditional Chinese medicine preparation high-dose group significantly decreased (P<0.01). <0.05); the hemagglutination titers of the lung tissue of the mice in each drug group were significantly decreased (P<0.01); the pathological morphology of the lung tissue of the mice in each drug group was improved to varying degrees.

　　Conclusion: The compound traditional Chinese medicine prescription used in this study can significantly reduce the average lung index and average blood coagulation titer of mice infected with influenza virus A/PR/8/H1N1, and can significantly improve the lung injury caused by it.