动物造模,高血压 z-bio 2022-07-06 319

　　Objective To investigate the effect of modified Wuzhuyu Decoction on blood pressure and vascular endothelial function in hypertensive model rats induced by high-sugar and high-fat diet and drinking.

　　Methods 100 SPF male SD rats were selected, 20 were normal control group, and 80 were used to prepare high-sugar and high-fat diet and gradient drinking hypertension model. The 80 successfully modeled rats were randomly divided into 4 groups: hypertension model group, modified Wuzhuyu Decoction low-dose treatment group, middle-dose treatment group and high-dose treatment group, with 20 rats in each group. The treatment group was given different concentrations of modified Wuzhuyu decoction by gavage every day, while the rats in the normal control group and the hypertensive model group were given distilled water by gavage. The systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MBP) and heart rate (HR) were measured before the experiment, after successful modeling (before administration) and after treatment. Enzyme-linked immunosorbent assay was used to determine serum intercellular adhesion molecule-1 (ICAM-1), endothelin-1 (ET-1), nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) before and after treatment. )Level.

　　Results Compared with the normal control group, the SBP, DBP and MBP of the rats in each treatment group were significantly increased after modeling (before administration) (P<0.05). Compared with the hypertension model group, the SBP, DBP and MBP of the rats treated with the middle-dose and high-dose modified Wuzhuyu decoction were significantly decreased (P<0.05). there="" was="" no="" significant="" difference="" in="" hr="" between="" groups="" of="" rats="" before="" and="" after="" treatment="" p="">0.05). Compared with the normal control group, the levels of ICAM-1 and ET-1 in each treatment group were significantly increased, while the levels of NO and eNOS were significantly decreased (P<0.05). The levels of ICAM-1 and ET-1 in the treatment group were significantly increased, while the levels of NO and eNOS were significantly decreased (P<0.05).

　　Conclusion Medium and high doses of modified Wuzhuyu decoction have obvious antihypertensive effects on hypertensive rats induced by high sugar, high fat and alcohol consumption.