动物造模 z-bio 2022-07-07 280

　　Objective To investigate the inhibitory effect of different concentrations of low molecular weight heparin on PICC-related thrombosis.

　　Methods 40 experimental rabbits were divided into group A (control group), group B (low-concentration low-molecular-weight heparin group), group C (medium-concentration low-molecular-weight heparin group), and D group (high-concentration low-molecular-weight heparin group) according to the random number table method. group), after the successful establishment of the PICC experimental rabbit model, 0.9% sodium chloride solution, 50 μ/(mL·d) low molecular weight heparin sodium solution, 100 μ/ (mL·d) low molecular weight heparin sodium solution, 200 μ/ (mL·d) low molecular weight heparin sodium solution was subcutaneously injected into the abdomen of the experimental rabbits, once a day for 7 consecutive days. The marginal ear vein, anterior vena cava and PICC catheter were cut for HE staining, and the incidence of CRT and coagulation function in different parts of the four groups of experimental rabbits were observed and compared under light microscope.

　　Results The incidence rate of CRT in group A was 100%, and there was no thrombosis in group D. There was a statistically significant difference in the incidence of thrombosis between group A and the other three groups (P<0.01). Among them, there were significant differences in the incidence of ear edge vein thrombosis between the experimental rabbits in group A, group B and group C compared with group D (P<0.01). The incidence of PICC catheter thrombosis between groups A and B was significantly different from that between groups C and D (P<0.01). One week after PICC intubation, the levels of blood PT, APTT, TT, FIB and D-D dimer were significantly different among the groups (P<0.01).

　　Conclusion Different concentrations of low molecular weight heparin have a preventive effect on the formation of CRT, and the effect increases with the increasing concentration. Among them, high concentration of low molecular weight heparin 200 μ/(mL·d) can effectively prevent the formation of CRT and reduce the incidence, which is of great importance. clinical significance.