动物造模 z-bio 2022-07-07 366

　　Objective To observe the expression of HE staining, VEGF (vascular endothelial growth factor) and FCF (fibroblast growth factor) in myocardial tissue sections induced by subcutaneous injection of isoproterenol (1 mg/kg body weight) for different days in rats with specific cardiomyopathy. and changes in ECG T waves.

　　Methods Except the blank group, the rats in each group were subcutaneously injected with isoproterenol (1 mg/kg) at multiple points. The group received subcutaneous injection of isoproterenol (1 mg/kg) for 4 days, and the group received continuous subcutaneous injection of isoproterenol (1 mg/kg) for 6 days. After 1 week of administration, the rats were anesthetized, electrocardiograms were measured, and the hearts were taken. HE staining was used to observe the changes in the degree of myocardial cell injury in the rats, and immunohistochemical staining was used to observe the myocardial tissue sections of VEGF (vascular endothelial growth factor), FGF (formation factor). fibroblast growth factor) expression.

　　Results Compared with the blank control group, the continuous subcutaneous injection of isoproterenol (1 mg/kg) at multiple points for 6 days showed significant differences in HE staining tissue sections under microscope. The expression of endothelial growth factor) and FCF (fibroblast growth factor) was also significantly different from that of the blank control group.

　　Conclusion Compared with the blank control group, the results of this study showed that the myocardial tissue sections of the model rats with specific cardiomyopathy induced by continuous subcutaneous multi-point injection of isoproterenol (1mg/kg) for 6 days had HE staining, VECF (vascular endothelial growth factor) , FGF (fibroblast growth factor) expression and the changes of ECG T wave have significant differences, so continuous subcutaneous injection of isoproterenol (1mg/kg) for 6 days in rats can be used as an experiment to evaluate the efficacy of specific cardiomyopathy Use of animal models.