动物造模 z-bio 2022-07-07 362

　　Objective To investigate the correlation between the expression of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in hippocampus and forebrain cortex and chronic stress-induced depression in mice.

　　Methods Fifty-eight SPF grade KM mice, 29 male and 29 male mice, were selected. The mice were randomly divided into the control group and the observation group. The mice in the observation group were stimulated by a variety of stress factors such as fasting and electric shock, and one of them was randomly selected every day for a total of 20 days. The mice in the control group did not receive the experimental stressors and were fed under the same conditions. The body weight of mice was measured before stress, 10d and 20d after stress. The two groups of mice were tested by Morris water maze test and open field test, and the results were observed and recorded. The expressions of BDNF and VEGF in the hippocampus and forebrain cortex of mice in each group were measured, and their correlations were analyzed.

　　Results Before stress, there was no significant difference in body weight between the two groups of mice (P>0.05). After stress, the weight of mice in the observation group was significantly lower than that in the control group (P<0.01). The escape latency, swimming distance and dwell time in the second quadrant were significantly higher in the observation group than in the control group (P<0.05 or P<0.01), while="" the="" dwell="" time="" in="" other="" quadrants="" was="" p="">0.05). The modification times and vertical movement scores of mice in the observation group were significantly lower than those in the control group, the stay time in the central area was significantly higher than that in the control group (P<0.01), and="" there="" was="" no="" significant="" difference="" in="" the="" number="" of="" defecation="" particles="" p="">0.05). Compared with the control group, the expression levels of BDNF and VEGF in the hippocampal CA1 area and forebrain cortex of the observation group were significantly decreased (P<0.05), but="" there="" was="" no="" significant="" difference="" in="" bdnf="" the="" ca3="" area="" between="" two="" groups="" p="">0.05).

　　Conclusion Chronic stress can lead to depression-like behavior in mice, which may be related to the decreased expression of BDNF and VEGF in the hippocampus and forebrain cortex.