动物造模 z-bio 2022-07-08 347

　　Objective To explore the effect of deletion of two type I interferon receptors on in vitro fertilization in mice and to optimize the conditions of in vitro fertilization.

　　Methods In vitro fertilization and embryo transfer were performed on two type I interferon receptor-deficient mice (IFN-α R- / - , IFN-α/β R- / - ) and background wild-type mice (C57BL/6), respectively. , 5 mice in each group were superovulated for 3 times, and the relevant data were recorded to analyze whether the deletion of interferon receptors has an effect on the in vitro fertilization of mice. In vitro fertilization hybridization experiments were carried out with the gametes of two kinds of mice with type I interferon receptor deletion and C57BL/6 mouse gametes, 5 mice in each group, with three repetitions to explore the effect of type I interferon receptor deletion on male and female gametes in vitro Fertilization effects. At the same time, the conditions of in vitro fertilization were optimized, and the technical methods to improve the fertilization rate were explored, with 5 mice in each group, repeated three times.

　　Results The average in vitro fertilization rate of the two type I interferon receptor-deficient mice was lower than that of the background strain C57BL/6 mice, and the difference between groups was significant (P<0.05). The in vitro fertilization rate of interferon α receptor-deficient mice was higher than that of interferon α and β receptor double-deficient mice, and the difference between groups was significant (P<0.05). The in vitro fertilization rates of sperm and C57BL/6 mouse oocytes from the two types of interferon receptor-deficient mice were higher than the in vitro fertilization rates of their oocytes and C57BL/6 mouse sperm, and the difference between the groups was significant (P<0 .05). In vitro fertilization rate can be improved by prolonging sperm capacitation time to 1 h or adding 1 mmol/L reduced glutathione (GSH) to capacitation fluid and insemination fluid, and the difference between the corresponding groups is significant (P<0). .05).

　　Conclusion The deletion of type I interferon receptor may lead to the decrease of in vitro fertilization rate of the corresponding strains of mice, and the effect on oocytes is more significant than that on sperm. By appropriately prolonging the time of sperm capacitation or changing the composition of capacitation and semen, it can be improved. In vitro fertilization rate.