动物造模 z-bio 2022-07-08 238

　　Objective To observe the effect of taurine on the expression of blood hormones and IGF gene in intrauterine growth restriction pregnant mice.

　　Methods Healthy SD rats were reared in a 2:1 ratio of male and female. Pregnant rats were randomly selected according to the order of pregnancy and divided into normal diet group (C), low protein diet group (M), and low protein diet + taurine group (MT). , normal feed + taurine group (T). There were 10 pregnant mice in each group. M and MT groups were fed with low-protein diet during pregnancy to establish models. On the 12th day of pregnancy, pregnant mice in C and M groups were given drinking water, and MT and T groups were given taurine 300 mg/( kg·d). The weight growth of pregnant mice was detected every week, and samples were taken on the 21st day of gestation to record the weight, number, and number of fetal mice; Elisa method was used to detect the serum growth hormone (GH), insulin growth factor-1 (IGF-1), insulin (INS) of pregnant mice. ), thyroxine (T3 and T4) levels, real-time quantitative PCR was used to detect liver growth hormone receptor (GHR), insulin growth factor 1 (IGF-1), insulin growth factor binding protein 1 (IGFBP-1) and insulin in pregnant mice Growth factor binding protein 3 (IGFBP-3) mRNA expression.

　　Results In the taurine-added group, the body weight, number and teratogenicity of the fetuses were significantly improved, the body weight of the pregnant mice increased significantly (P<0.05), the blood INS and T4 increased significantly (P<0.05), and the liver IGF-1 increased significantly (P<0.05). , IGFBP-3 mRNA expression was significantly increased (P < 0. 05), and IGFBP-1 mRNA was significantly decreased (P < 0. 01).

　　Conclusion Adding taurine can improve thyroid function and GH/IGF-1 axis disorder in low-protein pregnant mice, which may play an important role in relieving fetal uterine growth restriction.