动物造模 z-bio 2022-07-08 252

　　Objective To observe the effect of hydroxychloroquine on the distribution of lymphocytes in peripheral immune organs (spleen) of mice with systemic lupus erythematosus (TC mice).

　　Methods 30-week-old female TC mice were randomly assigned. The experimental group was given hydroxychloroquine by gavage [10 mg/(kg·d)], and the control group was given normal saline [10 mg/(kg·d)] by gavage. d)], a total of 5 weeks of treatment. The titer of anti-dsDNA antibody in peripheral blood of mice was detected by enzyme-linked immunosorbent assay (ELISA), the pathological changes of kidney tissue were detected by hematoxylin-eosin (HE) staining, and the proportion of spleen lymphocytes in mice was detected by flow cytometry .

　　Results Compared with the control group, the renal histopathological score of the experimental group was significantly lower than that of the control group (P<0.05); the proportion of spleen B lymphocytes and germinal center B lymphocytes in the experimental group was significantly lower than that of the control group (P<0.05). 0. 05); the proportion of spleen regulatory T lymphocytes (Treg) in the experimental group was significantly higher than that in the control group (P < 0. 05); there was no significant difference in the level of anti-dsDNA antibody and the proportion of Th17 lymphocytes between the two groups (P < 0. 05). >0.05).

　　Conclusion Hydroxychloroquine has a regulatory effect on the immune response of lymphocytes in peripheral immune organs of lupus mice and can alleviate the pathological damage of kidney tissue in TC mice.