动物造模,阿霉素致心力衰竭 z-bio 2022-07-08 322

　　Objective To investigate the effect of thrombopoietin (TPO) on cardiomyocyte apoptosis in rats with adriamycin-induced heart failure (CHF) and its regulatory mechanism on TGF-β1/Smad3 signal.

　　Methods Rats were randomly divided into control group, model group, treatment group and inhibitor group. The model group, treatment group and inhibitor group were given intraperitoneal injection of adriamycin to establish a heart failure model. The treatment group received intraperitoneal injection of 5 μg/kg TPO daily, and the inhibitor group received intraperitoneal injection of 1.2 mg/kg SB431542 daily. TUNEL staining, immunofluorescence, Western blot were used to detect myocardial cell apoptosis, Caspase-3, TGF-β1 and p- The expression level of Smad3.

　　Results Compared with the control group, the myocardial cell apoptosis rate and the expression of TGF-β1, p-Smad3 and Caspase-3 in the model group were significantly increased. The expressions of TGF-β1, p-Smad3 and Caspase-3 were significantly decreased, and the differences were statistically significant (P<0.05).

　　Conclusion TPO can reduce the apoptosis rate of cardiomyocytes induced by adriamycin in CHF rats, and its mechanism may be related to the inhibition of TGF-β1/Smad3 signaling.