动物造模 z-bio 2022-07-08 283

　　Objective To apply hypoxic training to obese mice to observe the changes of GHSR levels in gastric tissue and hypothalamus, so as to explore whether hypoxic training affects the Ghrelin-GHSR pathway to improve obesity.

　　Methods Male C57BL/6J mice were randomly divided into normal control group (C, n=8) and high-fat diet group (H, n=52). , obese normoxic exercise group (HE), obese hypoxic exposure group (HH) and obese hypoxic exercise group (HHE), each exercise group was given moderate-intensity treadmill training, and each hypoxic group was given intermittent hypoxia exposure (11. 2% oxygen content), recorded weekly body weight and food intake for 4 weeks, detected serum TC, TG, GLU and total Ghrelin levels, RT-PCR method to detect hypothalamic GHSR and gastric Ghrelin mRNA expression levels, WB to detect hypothalamic GHSR and Ghrelin levels. NPY protein content and gastric tissue Ghrelin, Goat and HIF-2α protein content.

　　Results (1) After 4 weeks of intervention, the body weight of the HE, HH and HHE groups was significantly lower than that of the HC group. At the initial stage of hypoxia intervention, the food intake of the HH and HHE groups decreased, but with the prolongation of the intervention time, the obesity groups decreased. (2) The blood indexes of the HC group were significantly higher than those of the C group, the TC level of the HH group was significantly lower than that of the HC group, the GLU levels of the HE, HH and HHE groups were significantly lower than those of the HC group, and the serum levels of the HH and HHE groups were significantly lower than those of the HC group. The total ghrelin level was significantly lower than that of the HC group. (3) The levels of hypothalamic GHSR mRNA and gastric ghrelin mRNA in the HC group were significantly lower than those in the C group. The levels of GHSR protein in the hypothalamus of the HE, HH and HHE groups were significantly higher than those of the HC group, the levels of NPY protein in the HE and HHE groups were significantly higher than those in the HC group, (5) the levels of gastric Ghrelin protein in the HE, HH and HHE groups were significantly higher than those in the HC group, The level of Goat protein in HE group and HHE group was significantly higher than that in HC group, and the level of HIF-2α protein in HH group and HHE group was significantly higher than that in HC group.

　　Conclusions Hypoxic training can reduce the body weight of obese mice by regulating the Ghrelin-GHSR signaling pathway and affecting glucose and lipid metabolism.