动物造模,心肌缺血再灌注损伤 z-bio 2022-07-08 302

　　Objective To investigate the protective effect and mechanism of activated protein C (APC) on myocardial ischemia-reperfusion injury in rats.

　　Methods Thirty male SD rats were randomly divided into sham operation group (10 rats), I/R group (10 rats) and APC treatment group (10 rats), the left anterior descending coronary artery was ligated for 60 min and reperfused for 6 h to prepare myocardium. In the ischemia-reperfusion model, ELISA and immunohistochemistry were used to detect the expression levels of TNF-α, MPO and ICM-1 in myocardial tissue respectively, and HE staining was used to detect the pathological changes of myocardial tissue in each group.

　　Results Compared with the sham operation group, the expression levels of TNF-α, MPO and ICM-1 in the I/R group were significantly increased (P<0.01). The expression levels of MPO and ICM-1 were significantly decreased (P<0.01), and the infiltration of inflammatory cells was observed under the light microscope, the most obvious in the I/R group, and the APC group was significantly less than the IR group.

　　Conclusion APC has a protective effect on myocardial ischemia-reperfusion injury, and its mechanism may be related to inhibiting the expression of inflammatory factors and reducing tissue inflammatory cell infiltration.