动物造模 z-bio 2022-07-11 269

　　OBJECTIVE: To study the effect of acute exercise on myocardial miRNA-30a and the expression of autophagy-related genes in rats, and to explore the regulatory mechanism of miRNA-30a on myocardial autophagy activity in different recovery periods after exercise.

　　Methods: SD rats were randomly divided into quiet control group (Con), 0h post-exercise group (E0), 3h post-exercise group (E3) and 12h post-exercise group (E12). Materials were collected at different time points after acute exercise. Using real-time The expression levels of ACE mRNA, miRNA-30a, Beclin-1 mRNA and LC3 mRNA in left ventricular myocardium were detected by fluorescence quantitative polymerase chain reaction (PCR).

　　RESULTS: Compared with the resting control group, the expressions of ACE mRNA and miRNA-30a in the left ventricular muscle of the exercise group at 0 h after exercise were significantly increased (P<0.05), and the expressions of Beclin-1 mRNA and LC3 mRNA were also significantly increased (P<0.05). All returned to a quiet level 3 hours after exercise.

　　Conclusion: Acute exercise can significantly increase the expression of myocardial ACE mRNA, miRNA30a, Beclin-1 mRNA and LC3 mRNA, and this transient increase may be caused by exercise stress.