动物造模 z-bio 2023-05-08 421

　　Objective: To observe the effect of the preparation "Fujiu Tie Gao" of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine on serum interleukin-4, interferon γ, related immunoglobulin E cytokines, nerve growth factor and tyrosine kinase A protein in the lung tissue of asthmatic mice. To explore the effect of its expression on the treatment of asthma.

　　Methods: Fifty adult BALB/c mice were randomly divided into 5 groups: A normal control group, B asthma model group, C dexamethasone group, D Fujiutie high-dose group, and E Fujiutie low-dose group. Ovalbumin Sensitized by intraperitoneal injection, challenged by ultrasonic nebulization inhalation, and established asthma model. ELISA method was used to detect the expression of IL-4, total IGE, OVA-specific IGE and IFN-γ cytokines in serum, HE staining and Masson staining were used to observe the pathological changes, immune The expression of NGF and TrKA protein in lung tissue was observed by histochemistry.

　　Results: Compared with the normal group, the expression of NGF, TrkA and IL-4, total IGE and OVA-specific IGE in the model group were significantly increased (P<0.05), and the expression of IFN-γ was decreased (P<0.05); Compared with the dexamethasone group and the Fujiutie high-dose group, the expressions of NGF, TrkA, IL-4, total IGE, and OVA-specific IGE were decreased (P<0.05), and the expression of IFN-γ was increased (P<0.05). volt="" p="">0.05).

　　Conclusion: Fujiu plaster can inhibit the secretion of IL-4 and IGE by down-regulating the expression of NGF and TrKA proteins, and at the same time promote the secretion of IFN-γ, so that TH1 and TH2 tend to balance, thereby relieving asthma attacks.