动物造模 z-bio 2022-07-12 354

　　Objective: To find the specific targets of UC with spleen and kidney yang deficiency.

　　Methods: Ninety-six Wistar rats were randomly divided into model group, high-dose group, medium-dose group, low-dose group, and SASP group, and the treatment group was given corresponding drugs by gavage. Colon tissue of the rats in the blank group and rats in the model group were selected respectively. High-throughput sequencing was performed on the colon tissue at the lesion site. The gene expression of the screened chemokines was detected by RT-qPCR.

　　RESULTS: Compared with the model group, the differentially expressed genes of the blank group were screened according to q⁃value≦0.0 fold⁃change≧1.5. The GO functional classification analysis showed that the differential gene functions were mainly enriched in biological processes. , BP), cellular component (CC), and molecular function (MF). Through differential gene KEGG enrichment analysis, it was found that CXCL1, CXCL2, CXCR2, CXCL6, CCL7, and CCL12 gene expression in the chemokine signaling pathway It was verified by RT-qPCR that the gene expression changes of the above factors were consistent with the sequencing results.

　　Conclusion: CXCL1, CXCL2, CXCR2, CXCL6, CCL7, CCL12 gene expression in the chemokine signaling pathway of spleen-kidney yang deficiency type ulcerative colitis is significantly up-regulated, which can be used as an objective indicator of UC mucosal inflammatory activity. Decoction and Sishen Pill Compound Chinese Medicine Granules can effectively down-regulate the expression of the above factors, slow down the inflammatory response, and promote the repair of the damaged colonic mucosa.