动物造模 z-bio 2022-07-13 266

　　Objective: To investigate the changes of ovarian function in female mice by the combined intervention of calcium, magnesium, copper, zinc, vitamin D and protein on coal-burning fluorosis.

　　Methods: 120 clean-grade SD female rats weighing about 80 g were selected as the research objects, and were randomly divided into the control group, the fluoride dyeing group, the multi-element group, and the multi-element + protein group, with 30 rats in each group. Rats in each model group were given free access to different formulas of raw coal and corn feed mixed with peat. The female mice were sacrificed at 60, 120, and 180 days after exposure to fluoride, respectively, and the bilateral ovaries were isolated. The expression of apoptosis proteins Bcl-2 and Bax in ovarian granulosa cells was detected by immunohistochemistry. The morphology of ovarian tissue and the number of follicles at all levels were observed under the HE staining light microscope.

　　RESULTS: There was no significant difference in the expression of Bcl-2 protein in ovarian granulosa cells. Compared with the fluoride exposure group, the Bax protein of each group was significantly different at 120 d and 180 d (P < 0.05), and the difference between the two intervention groups at 180 d was significant (P < 0.05). Compared with each group, the expression of Bax protein in the fluoride exposure group gradually increased, and the difference was significant (P < 0.05). There were significant differences between 60d and 180d for various element groups (P < 0.05). In the fluoride exposure group, the ovarian granulosa cells showed progressive edema, and the atresia follicles and corpus luteum degenerated gradually increased. Ovarian granulosa cells were rare and mild edema in the two intervention groups, normal follicles at all levels were more common, and atretic follicles and degenerated corpus luteum were significantly reduced; compared with the fluoride exposure group, the atretic follicles in the 60-day control group and the multiple elements + protein group decreased. , the difference was significant (P < 0.05); the 120-day and 180-day control group and the two intervention groups had significant differences (P < 0.05).

　　Conclusion: Calcium, magnesium, copper, zinc and vitamin D can antagonize the reproductive toxicity of coal-fired fluorosis on the ovary, reduce the expression of Bax protein, and reduce the number of atretic follicles, and the combined intervention effect of adding protein is better.