动物造模,骨质疏松 z-bio 2022-07-13 213

　　Objective: To observe the effect of osteopeptide for injection on bone mineral density, bone biomechanics and mRNA expression of calcium-binding protein (CaBp-D9K) in ovariectomized osteoporosis rats, and to explore the mechanism of osteopeptide for injection in the treatment of ovariectomized osteoporosis .

　　Methods: Forty-eight rats that were successfully modeled (with double ovariectomy) were randomly divided into the observation group and the model group, and another 24 rats were selected as the sham operation group (resection of part of the small mesentery) and the blank group. The blank group, sham operation group and model group were given normal saline by gavage, and the observation group was given 1.1 mg/kg of osteopeptide for injection by gavage. After 2 months of intervention, the bone volume (BV), trabecular bone thickness (Tb.Th), trabecular bone number (Tb.N), trabecular bone separation (Tb.Sp), bone mineral density, bone trabeculae were observed in the four groups. Biomechanics, serum 1,25(OH)2D3 and intestinal CaBp-D9K mRNA expression levels.

　　Result: BMD, maximum load, fracture load, BV, Tb. Th, Tb. N, serum 1,25(OH)2D3 and intestinal CaBp-D9K mRNA expression levels were lower than those in blank group and sham operation group, Tb. Sp was higher than the blank group and the sham operation group, and there was a significant difference between the groups (P < 0.05). Bone mineral density, maximum load, breaking load, BV, Tb. Th, Tb. N, serum 1,25(OH)2D3 and intestinal CaBp-D9K mRNA expression levels were higher than those in the model group, Tb. Sp was lower than the model group, and the difference between groups was significant (P < 0.05).

　　Conclusion: Osteopeptide for injection can reduce the degree of osteoporosis in ovariectomized rats, increase the expression of CaBp-D9k mRNA in the small intestine, and promote intestinal calcium absorption, which may be its important mechanism.