动物造模,肠易激综合征 z-bio 2022-07-13 248

　　OBJECTIVE: To find biological markers for the diagnosis and treatment of irritable bowel syndrome.

　　Methods: The post-inflammatory irritable bowel syndrome (PI-IBS) animal model was established by acetic acid plus restraint stress method; the colon motility index (MI), the number of fecal particles excreted within 2 h and the excretion time of glass pellets were used to evaluate The motility of the colon of rats was observed; the formation of PI-IBS model rats and the therapeutic effect of Wuji Pill on it were observed; the calcitonin gene-related peptide (CGRP) in the brain and colon tissue of PI-IBS rats was detected by ELISA. , motilin (MTL), neuropeptide Y (NPY), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), cholecystokinin (CCK) levels.

　　RESULTS: The PI-IBS rat model was successfully established. The body weight of model rats decreased; food intake decreased; defecation increased; loose stools and amorphous soft stools; voluntary exercise decreased; colonic MI increased significantly (P ＜ 0.05); the number of fecal particles excreted by rats increased significantly (P < 0.05); the discharge time of glass pellets was significantly shortened (P < 0.05). Wuji Pills can significantly improve the above symptoms after 7 days of treatment. Compared with the normal control group, the levels of CGRP, SS and VIP in the brain tissue of PI-IBS rats were significantly increased (P ＜ 0.05), and the concentration of NPY was significantly decreased (P ＜ 0.05). It can significantly reduce the concentration levels of CGRP, SS and VIP (P < 0.05), and significantly increase the concentration level of NPY (P < 0.05). Compared with the normal control group, CCK, NPY, MTL, SS and VIP in the colon tissue of PI-IBS rats were significantly decreased (P < 0.05); Wuji pills could significantly increase the levels of CCK and VIP (P < 0.05). P < 0.05).

　　Conclusion: Wuji Pills can be used to treat IBS by regulating the levels of various brain-gut peptides in the brain and colon tissue of IBS rats. These regulated abnormally-changed brain-gut peptides can become potential biological agents for the diagnosis and treatment of IBS. index.