动物造模 z-bio 2022-07-13 381

　　Objective To study the radioprotective effect of diallyl disulfide on mouse spermatogenic cells.

　　Methods An animal model of radiation injury was established by X-ray at a dose of 4 Gy. The changes of testis tissue, sperm motility and sperm deformity rate of irradiated mice were observed, and protein carbonyl content, malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-hydroxydeoxyguanosine) were detected. OHdG) content to evaluate the degree of radiation damage to spermatogenic cells and the protective effect of DADS on spermatogenic cells. Testicular tissue antioxidant enzymes were determined as superoxide dismutase (SOD), catalase (CAT), glutathione Peptide peroxidase (GSH-Px) activity and glutathione (GSH) content were detected, and the expression of Nrf2 signaling protein was detected by western blot to explore the radiation protection mechanism of DADS.

　　Results Compared with the simple irradiation group, the DADS pretreatment group had less damage to the testis tissue, significantly increased sperm motility (P<0.05), decreased sperm deformity rate (P<0.05), and significantly decreased protein carbonyl content, MDA and 8-OHdG levels. (P < 0.05). Antioxidative indexes such as SOD, CAT, GSH-Px and GSH were significantly increased (P < 0.05), and the expression of Nrf2 was significantly enhanced.

　　Conclusion DADS has a certain protective effect on acute radiation injury of spermatogenic cells in male mice through its antioxidant capacity.