动物造模 z-bio 2022-07-13 301

　　OBJECTIVE: To explore a fast, convenient and efficient new method for extracting rat dorsal root ganglia—reverse the direction of nerve running and extracting DRG.

　　Methods: The traditional method and the new method of opening the intervertebral foramen were used to extract DRG, and the time consumed by the two methods and the number of complete DRG extracted were compared.

　　RESULTS: The number of complete dorsal root ganglia of L3-5 segments extracted from each rat was (3.08 ± 1.31) by the traditional method, and the average time-consuming of each DRG was (5.58 ± 1.21) min. ; Using the new method, the number of complete dorsal root ganglia in L3-5 segments was (4.29 ± 1.08), and the average time per DRG was (1.69 ± 0.91) min. Compared with the traditional method, the difference was significant (P < 0.05).

　　Conclusion: The new method of searching and extracting DRG in the reverse direction of nerve running can more efficiently extract the complete rat dorsal root ganglion, which provides a more reliable method for the culture and morphological study of dorsal root ganglion cells in the later stage of the experiment. tissue material.