动物造模 z-bio 2022-07-15 403

　　OBJECTIVE: To investigate the protective effect of curcumin pretreatment on the intestinal mucosa of rats under the dry and hot desert environment.

　　Methods: Eighty male SPF rats were randomly divided into 40 normal saline control group (NC group) and 40 curcumin pretreatment group (HDC group). The NC group was intragastrically administered with normal saline, and the HDC group was intragastrically administered with 200 mg/kg curcumin for 7 consecutive days, once a day. The rats in each group were placed in an experimental chamber simulating a dry and hot desert environment, temperature: (41±0.5) ℃, relative humidity: (10±2)%. At the time points of 0, 50, 100, and 150 min, the rats in the NC group and HDC group (10 in each group) were randomly taken out, respectively, and anesthetized by intraperitoneal injection of sodium pentobarbital, and the ileal samples were collected for pathological observation, scoring and oxidation Detection of stress indicators CAT, SOD, MDA.

　　Results: At 0 and 50 min, the pathological damage score of HDC group was not significantly different from that of NC group (P>0.05); at 100 and 150 min, the pathological damage score of HDC group was significantly lower than that of NC group (P<0.01). ). At 50, 100, and 150 min, the activities of CAT and SOD in HDC group were significantly higher than those in NC group (P<0.05 or P<0.01), and the content of MDA in HDC group was significantly lower than that in NC group (P<0.05 or P<0.01). . After exposure to dry heat, the intestinal mucosal pathological damage score in the NC group was negatively correlated with the activities of CAT and SOD (r=-0.9128, r=-0.9125, both P<0.01), and positively correlated with the MDA content (r=0.9258, P<0.01). < 0.01).

　　CONCLUSION: Curcumin pretreatment has a protective effect on the intestinal mucosa of rats under the dry and hot desert environment, and the mechanism may be related to curcumin's inhibition of intestinal mucosal oxidative stress.