动物造模,脓毒症 z-bio 2022-07-15 323

　　Objective: To explore the effect of oat β-glucan on the expression of genes related to small intestinal injury in sepsis.

　　Methods: SD rats were randomly divided into normal control group (NC group), experimental control group (TC group), and oat β-glucan group (Oglu group). The sepsis model was established by cecal ligation and puncture in the TC group and the Oglu group. Oglu group was given different doses of oat beta-glucan by gavage. After 12 h, the small intestine tissues of each group were collected, and Western blot was used to detect the expression of small intestinal tissue injury genes.

　　Results: 1) Oat β-glucan attenuated sepsis-induced small intestinal injury, and decreased the levels of TNF-α, IL-1β and IL-6 in the small intestinal tissue of sepsis rats (P < 0.05); (2) Pus The expression of small intestine injury-related protein Bcl-2 in the toxic group was down-regulated, and the expression of Bax, caspase-3, Toll-like, apoptosis genes FAS and FASL, and NF-κB were significantly increased; (3) The small intestinal mucosa Bax, The expressions of caspase-3, Toll-like, apoptotic genes FAS and FASL, and NF-κB were lower than those in the obvious sepsis group, while the expression of Bcl-2 was higher.

　　Conclusion: Oat β-glucan regulates the expression of small intestinal injury genes in septic rats, and protects the small intestinal epithelium from sepsis injury.