动物造模,接触性皮炎 z-bio 2022-07-15 262

　　Objective: To study the therapeutic effect of sea buckthorn oil on allergic contact dermatitis in mice.

　　Methods: 60 ICR mice were randomly divided into 6 groups: blank control group, model control group, seabuckthorn oil topical group (100%, 50% and 25%) with three doses of high, medium and low doses, and positive drug control group (compound dexamethasone acetate) metasone cream), 10 in each group. The mouse ACD model was established by sensitization and challenge with 2,4-dinitrochlorobenzene (DNCB), and the ear skin lesion score was used as an indicator for the success of the model establishment. The swelling degree and spleen index were measured, and hematoxylin and eosin (HE) staining was used to observe the pathological changes such as inflammatory cell infiltration in the ear skin of mice.

　　Results: The measurement results of ear swelling in mice showed that the high-dose seabuckthorn oil group (9 mL/kg, for external use) could significantly inhibit the DNCB-induced ear swelling in mice, and the difference was significant compared with the model group (P<0.01), and="" the="" there="" was="" no="" significant="" difference="" in="" positive="" control="" group="" p="">0.05). The results of the mouse spleen index measurement indicated that each dose group of sea buckthorn oil may play a certain immunoregulatory effect by promoting the development of immune organs. The results of pathological examination showed that compared with the model group, the thickness of the ear tissue in each dose group of seabuckthorn oil was reduced, the edema of the epidermis and dermis was significantly reduced, the infiltration of lymphocytes was significantly redu