动物造模 z-bio 2022-07-15 263

　　OBJECTIVE: To investigate the effects of 80-day-old offspring on cognitive development and the content of dopamine and dopamine in hippocampus, and to explore the correlation between the two.

　　METHODS: The pregnant rat model of fear of kidney injury was established by the spectator electric shock method. The Morris water maze test was performed on the 80-day-old offspring to observe their cognitive development. Liquid chromatography-electron capture detection (HPLC-ECD) method was used to determine the contents of DA and DOPAC in the brain dialysate of offspring mice.

　　Results: Compared with the control group, the average escape latency time of the 80-day-old model group increased, the swimming speed decreased, the residence time in the 20% edge area increased, and the number of crossings decreased, with significant differences (P<0.05); 80 days The levels of DA and DOPAC in the extracellular fluid of the hippocampus of the offspring of the model group were decreased at each perfusion time point, and the difference was significant (P < 0.05). There was a positive correlation between the escape latency and the number of crossing stations, and a negative correlation with the residence time in the 20% marginal area, and the correlation was significant (P<0.05). 0.05).

　　Conclusion: Fear of injury to pregnant mice can affect the spatial learning and memory ability of 80-day-old offspring, and reduce the content of DA and DOPAC in the hippocampus. The level of cognitive development is closely related to the decrease in the content of DA and DOPAC in the hippocampus.