动物造模 z-bio 2022-07-18 352

　　OBJECTIVE: To study the changes of metabolic pathways during the immunosuppressive effect of cyclophosphamide in mice by means of metabolomics.

　　Methods: Flow cytometry was used to understand the changes of immune cells, and multiple results were discriminated by establishing a partial least squares model. Potential biomarkers were screened by variable importance in projection (VIP) parameters in the partial least squares model, and differential metabolites were identified by statistical analysis. Sexual metabolites were further identified and analyzed, 9 differential metabolites were finally identified through database search and operation, and 3 differential metabolic pathways were analyzed by pathway enrichment results.

　　Results: Cyclophosphamide had obvious effects on the biosynthesis of unsaturated fatty acids, mitochondrial fatty acid metabolism, glycerophospholipid metabolism, steroid hormone biosynthesis, arachidonic acid metabolism, fatty acid metabolism, and pyrimidine biosynthesis in normal organisms. influences.

　　Conclusion: Cyclophosphamide affects the most important metabolic pathways in normal group and experimental group are arachidonic acid metabolism, glycerophospholipid metabolism and pyrimidine metabolism.