动物造模 z-bio 2022-07-19 481

　　Objective: To explore the role of endoplasmic reticulum protein SEL1L in maintaining normal physiological activities of mammalian central nervous system.

　　Methods: Using Cre/loxp technology, neuron (neuron)-specific SEL1L knockout mice (NKO) were constructed and divided into experimental group (NKO) and wild-type group (WT) according to different genders, with 10 mice in each group. The differences in behavioral indicators such as survival time, body weight, grasping force, balance coordination, movement, and anxiety of mice at different ages after birth were compared and analyzed, and the physiological function of SEL1L in the central nervous system was judged.

　　Results: The survival time of NKO mice was (6±3) weeks; their body weights were 54.64%, 40.54%, and 38.57% of those of WT mice at the 3rd, 5th, and 8th weeks, respectively; At the 3rd, 5th, and 8th weeks, the force was 44.24%, 48.09%, and 49.04% of the WT male mice, and the NKO female mice were 39.39%, 50.19%, and 49.69% of the WT female mice. The exercise time of the balance and coordination of the NKO male mice was 26.92%, 41.58%, 37.48% of the WT mice, and the NKO female mice were 46.02%, 47.67%, 38.48% of the WT mice; the third The movement distance and the time to enter the central area of NKO male mice at 5 and 8 weeks in the open field test were (24.63%, 9.57%), (25.87%, 11.63%), (51.96%, 9.97%) of WT mice, respectively. ), NKO female mice were (35.62%, 25.93%), (42.75%, 9.77%), (34.77%, 14.49%) of WT mice, and showed obvious anxiety. The difference was significant (P<0.001).

　　Conclusion: NKO mice are significantly worse than wild-type mice in terms of survival time, body weight, balance and coordination, exercise, anxiety and other behavioral indicators, indicating that SEL1L protein is necessary to maintain the normal function of the central nervous system.