动物造模 z-bio 2023-02-16 480

　　Objective: To investigate the effect of recombinant human parathyroid hormone (rhPTH) on fracture healing in 2-month-old male rats using Micro-CT.

　　Methods: Two-month-old male SD rats were used to establish unilateral closed femoral fracture internal fixation model, and they were randomly divided into a parathyroid hormone treatment group and a control group, and were injected subcutaneously with recombinant human parathyroid hormone (1-34) after surgery. 10 μg/(kg.d) or equal dose of normal saline placebo (control group), bilateral femur specimens were taken on the 2nd, 7th, 14th, 21st and 42nd postoperative day, respectively, and X-ray examination and Micro-CT were performed respectively. Observation and quantitative analysis of bone structure were performed.

　　Results: On the 21st day after operation, the continuity between the callus on both sides of the fracture end in the parathyroid hormone group was good, and the fracture line was blurred. Compared with the control group, the parathyroid group had bone volume (BV), bone volume fraction (BV/TV), bone mineral density (BMD) and trabecular bone pattern factor (trabecular bone) on the 42nd postoperative day. pattern factor, Tb.Pf) was significantly higher than that of the control group; while the trabecular separation (Tb.Sp) and the degree of anisotropy (DA) were lower than those of the control group.

　　Conclusion: Low-dose recombinant human parathyroid hormone can increase BV, BV/TV, Tb.Pf and BMD and decrease Tb.Sp and DA of fractures in rats, thereby promoting fracture healing.