动物造模 z-bio 2022-12-17 480

　　Objective: To express and purify asprosin protein in prokaryotic cells of Escherichia coli, and to study its effect on the improvement of cardiac function.

　　Methods: The asprosin coding sequence was obtained from GenBank, codon-optimized according to the codon preference of Escherichia coli, the whole gene was synthesized, connected to the expression vector, and the expression was induced and purified by IPTG. By ligating and then relaxing the left anterior descending coronary artery, a mouse model of cardiac function injury was established. Thirty mice were randomly divided into 3 groups: sham operation group (sham), cardiac function impairment group (MI/R) and cardiac function impairment group (MI/R+rAsp) injected with recombinant protein. The left ventricular function was detected by echocardiography to evaluate the degree of cardiac function damage, and then to study the effect of Asprosin on the improvement of cardiac function.

　　Result: After prokaryotic expression and purification, the purity of the target protein is greater than 95%, and the endotoxin content is less than<0.1 EU/μg protein, which is suitable for cell and animal research. The cardiac function injury model was successfully established. Compared with the simple injury group, the cardiac function of the mice was significantly improved after exogenous administration of recombinant asprosin protein (P<0.05).

　　Conclusion: Asprosin protein can inhibit the damage of cardiac function and improve cardiac function.