动物造模 z-bio 2022-07-19 294

　　Objective: To screen the vacant chromosome 12 markers in the local standard for genetic quality control of Beijing miniature pigs DB11/T828.3-2011, and to analyze the applicability of landmarks by comparing the genetic evaluation of different markers in the same population.

　　Methods: Combined with literature reports, 4 pairs of microsatellite markers on chromosome 12 were selected and screened by 2 Chinese agricultural and large pig populations; the highly polymorphic chromosome 12 markers were selected and 18 pairs of chromosome markers of the standard method were selected to form a complete chromosome. The method was used to compare and analyze the genetic quality evaluation of the same batch of population through different methods. The obtained results were processed and analyzed using Popgen32 software.

　　RESULTS: The PIC values of the 4 pairs of chromosome 12 markers screened were all greater than 0.5, indicating high polymorphism; the current standards in the three indicators of chromosome coverage, amplification stability, and result evaluation were 94.7%, 96.0%, and Nongda I respectively. The line was unqualified but the third line of Agricultural University was qualified. The results of the three indicators evaluated after adding chromosome 12 were 100%, 96.6% and both groups were qualified.

　　Conclusion: The four pairs of chromosome 12 markers screened in this study are highly polymorphic, which provides data support for the improvement of DB11/T828.3-2011.