动物造模,自身免疫性脑脊髓炎 z-bio 2023-03-02 569

　　Objective: To investigate the effect of paclitaxel on the expression of CD28 and CTLA-4 in the brain tissue of rats with experimental autoimmune encephalomyelitis and the content of B cell stimulating factor (BAFF) in the supernatant of spleen tissue and its significance.

　　Methods: Fifty Wistar rats were randomly divided into 5 groups: paclitaxel (PTX) small, medium and large dose groups (PTX doses were 1 mg/kg, 2 mg/kg, 4 mg/kg, respectively) , normal control group, EAE control group, 10 in each group. The guinea pig spinal cord was used to make GPSCH and mixed with CFA in equal volume to make immune antigen, which was injected into the bilateral footpads of rats (2 mL/kg) for EAE modeling. 10 days. The normal control group and the EAE control group were given intraperitoneal injection of 0.9% NaCl 2 mL. Brain histological scoring was used to evaluate the inflammatory infiltration in the brain tissue of the experimental rats. The rat brain tissue was taken to measure the content of CD28 and CTLA-4 in the brain tissue by flow cytometry. The spleen tissue was taken and made into supernatant, and the spleen tissue was measured by ELISA Medium BAFF content.

　　Results: The brain tissue scores of each PTX dose group were lower than that of the EAE control group, and the difference between the groups was significant (P<0.01). The difference was significant (P < 0.01); the expression of CTLA-4 was higher than that of the EAE control group, and the difference between the groups was significant (P < 0.01); the content of BAFF in the spleen tissue supernatant was lower than that of the EAE control group. The difference between groups was significant (P < 0.01).

　　Conclusion: PTX can reduce the neurological dysfunction score of EAE rats, and its mechanism may involve regulating the expression of CD28 and CTLA-4 in the brain tissue of rats and the content of BAFF in the supernatant of spleen tissue, thereby exerting a preventive effect on EAE.