动物造模,肾缺血再灌注损伤 z-bio 2022-07-19 273

　　OBJECTIVE: To investigate the sensitivity and feasibility of ultrasonic radiation force to promote the adhesion of targeted microbubbles to improve the detection of renal ischemia-reperfusion injury.

　　Methods: Mouse renal ischemia-reperfusion model and normal control group were established, and they were divided into IR1h, IR3 h, IR6 h, IR12 h, and IR24 h groups according to 1, 3, 6, 12, and 24 h after reperfusion. Each group was randomly divided into a single-click targeted microbubble (MBICAM) group and a targeted microbubble + ultrasonic radiation force (MBICAM + USRF) group according to the presence or absence of ultrasonic radiation force.

　　RESULTS: In both the MBICAM group and the MBICAM+USRF group, there was no obvious contrast enhancement in the kidneys of the control group, and there was no significant difference in the renal acoustic intensity (VI) value (6.47±0.42 vs. 6.45±0.62, P=0.923). The imaging enhancement can be seen in different time windows of renal IR injury, and the imaging intensity gradually increases with the passage of time. Compared with the MBICAM group, the renal VI value in each time window of ischemia-reperfusion in the MBICAM+USRF group was significantly higher than that in the MBICAM group, and there was a significant difference between the two (P=0.000). There was no significant difference between IR12 h and IR24 hVI values (P=1.000), and there were significant differences among the other groups (P<0.05).

　　Conclusion: The application of ultrasonic radiation force combined with targeting microbubbles carrying anti-intercellular adhesion molecule-1 monoclonal antibody can improve the sensitivity of detection of renal ischemia-reperfusion injury in mice, and can be used for early evaluation of microvascular inflammation or related vascular endothelial response.