脱核酸酶,动物基因干扰 z-bo 2020-08-19 450

　　CRISPR interference or CRISPR-on uses denuclease activity Cas9 (dCas9) and transcription effect fusion protein and a variety of specific guide RNA (gRNA)-dCas9 fusion systems to block or activate the transcription technology of target genes. The Liu Dong research team of the School of Life Sciences published a research paper titled "Non-catalytic Cas9-mediated gene regulation of transcriptional activity in Caenorhabditis elegans and Clostridium reesei" in the journal Cell Research on March 27.

　　The fusion system can effectively change the development of the inner ear of zebrafish and the expression of genes related to nematode resistance. This method is not only suitable for the expression regulation of protein-coding genes, but also theoretically can be used for the regulation of non-coding RNA expression and modification of special DNA sites. Below method. From

　　Long Lijiang (PTN) PhD students Guo Hong and Yao Di of the research group are the co-lead authors of this article. Researcher Liu Dong and Dr. Liu Pengpeng are corresponding authors. This work was jointly completed by the National Natural Science Foundation of China, the 973 project of the Ministry of Science and Technology, the National Institute of Biomembrane and Membrane Biotechnology, the Cell and Proliferation Institute of the Ministry of Education, and the Peking University-Tsinghua Life Sciences Joint Center.