动物造模 z-bio 2022-07-20 426

　　Objective: To investigate the role of macrophage NF-κB activating factor 1 in inflammatory bowel disease.

　　Methods: The mice whose macrophage Act1 expression was inhibited by targeted inhibition (anti-Act1) were used as the research objects, and the ulcerative colitis model was induced by dextran sodium sulfate (DSS). , blood in the stool, stool viscosity, etc. were scored for disease activity index, colorectal tissue was stained with HE to evaluate the severity of inflammation, the infiltration of leukocytes and macrophages was analyzed by immunohistochemistry, and the related cells were detected by RT-qPCR. Changes in factor expression.

　　Results: Compared with C57 mice after DSS induction for 7 days, anti-Act1 mice had lower scores of colorectal inflammation indexes, less infiltration numbers such as macrophages, and mRNA expressions of TNF-α, IL-1β and IL-6. lower.

　　Conclusion: Targeted inhibition of Act1 expression in macrophages can alleviate DSS-induced ulcerative colitis.