动物造模 z-bio 2022-07-20 279

　　Objective: To explore the effect of benzene on liver and kidney function and blood nucleotide levels in Wistar rats.

　　Methods: Wistar rats were given different concentrations of benzene for 21 days, the levels of different blood components were detected by automatic biochemical analyzer, and the levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) and heat shock in blood were detected by enzyme-linked immunosorbent assay. Levels of protein-70 (HSP-70).

　　Results: Wistar rats were given different benzene doses of 0.19, 0.38, and 0.76 g/(kg·bw) for 21 consecutive days. Compared with the control group, the levels of total bilirubin in animal plasma increased by 134.40%, 173.63%, and 254.75%. Transaminases increased by 70.76%, 85.44%, 106.61%, creatinine increased by 24.54%, 67.46%, 84.50%, creatine kinase increased by 151.35%, 180.85%, 245.54%, urea [0.38, 0.76 g/(kg] bw) group] increased by 0.48% and 23.43%; the level of alanine transferase exceeded the upper limit of the detection value, both were 1185.60%; 31.29%, 40.46%, 272.14% higher, HSP-70 increased by 82.11%, 187.37%, 1484.21%; amylase levels were decreased by 62.03%, 63.66%, 64.45% compared with the control group.

　　CONCLUSION: Benzene pollution changes the ratio of cAMP and cGMP in blood, causes metabolic disorder, and leads to liver and kidney function damage or lesions.