动物造模 z-bio 2022-07-20 343

　　Objective: To investigate the effect of astragalus injection (AI) on the apoptosis of hippocampal neurons in hypoxia-hypoglycemia/reoxygenation and reglycemia (H/R) rats under the action of Bcl-2 inhibitor (TW-37).

　　Methods: The 8-day-old fetal rat hippocampal neurons were randomly divided into 6 groups: normal control group, H/R group, AI group, AI solvent control group, Bcl-2 inhibitor (TW-37)+H /R group, TW-37+AI+H/R group. Except for the normal control group, each group was subjected to hypoxia and hypoglycemia for 0.5 h, and then at seven time points (0 h, 0.5 h, 2 h, 6 h, 24 h, 72 h, 120 h) after reoxygenation and reglycemia. Check the indicators separately. The cell viability was detected by MTT assay, and the expression of Caspase-3 protein and mRNA in hippocampal neurons was detected by western blot and RT-PCR.

　　Results: Compared with the normal control group, except for 0 h, the activity of hippocampal neurons in the H/R group was significantly decreased (P<0.05), and the expressions of Caspase-3 protein and Caspase-3 mRNA were significantly increased (P<0.05). Compared with the /R group, except for 0 h, the activity of hippocampal neurons in the AI group was significantly increased (P<0.05), and the expression of Caspase-3 protein and mRNA were significantly decreased (P<0.05). h="" p="">0.05).

　　Conclusion: Astragalus injection can enhance the activity of hippocampal neurons in H/R rats and reduce the expression of Caspase-3 by activating the anti-apoptotic mechanism of Bcl-2. one of the important targets.