动物造模,急性肺水肿 z-bio 2022-07-20 374

　　Objective: To investigate the changes and significance of malondialdehyde, superoxide dismutase and interleukin-6 in lung tissue of acute hypoxia-induced acute pulmonary edema in rats.

　　Methods: Fifty adult Wistar rats were randomly divided into group A (normal group), group B (acute pulmonary edema model, hypoxia for 24 h), group C (acute pulmonary edema model, hypoxia for 48 h), group D ( Acute pulmonary edema model, hypoxia for 72 h) and group E (acute pulmonary edema hormone treatment group, hypoxia for 72 h). The acute pulmonary edema model was established by intraperitoneal injection of 6% ammonium chloride to the rats in groups B, C, D and E. The rats in group E were given 6% ammonium chloride 30 min after injection through the tail vein, and dexamethasone 6.0 mg/ kg. 24 h, 48 h, and 72 h after the successful modeling, the rats in 4 groups were sacrificed respectively, the rat plasma was separated, the rat lungs were harvested, and 10% lung tissue homogenate was prepared. MDA and SOD in lung tissue were detected by ELISA. IL-6 and plasma IL-6 content and activity.

　　RESULTS: Group B, C, and D showed obvious edema in the lung tissue of rats, and the wet weight increased significantly. Compared with group A, the difference was statistically significant (P<0.05). there="" was="" no="" obvious="" congestion="" and="" edema="" in="" the="" lung="" tissue="" of="" rats="" group="" shape="" generally="" normal.="" d="" rat="" model="" interstitial="" alveoli="" bronchioles="" are="" filled="" with="" protein-containing="" a="" hyaline="" membrane="" is="" formed="" alveoli.="" were="" most="" obvious.="" whole="" e="" lighter.="" he="" staining="" showed="" that="" interval="" fluid="" had="" been="" small="" amount="" eosinophilic="" scattered="" compared="" il-6="" increased="" sod="" decreased="" but="" difference="" not="" statistically="" significant="" p="">0.05). Compared with group A, MDA increased, SOD decreased, and IL-6 in plasma increased, and the difference was statistically significant (P<0.05). Compared with group D, MDA in lung tissue of rats in group E decreased, SOD increased, and IL-6 in plasma decreased, and the difference was statistically significant (P<0.05).

　　Conclusion: The occurrence of acute pulmonary edema is related to oxidative stress. The decrease of the body's antioxidant capacity and the increase of free radicals are important mechanisms for the occurrence of pulmonary edema. The contents of IL-6, SOD and MDA in the lung tissue have guiding significance for the change of the disease.