动物造模 z-bio 2022-07-20 313

　　OBJECTIVE: To investigate the role of telomerase in the formation and development of precancerous lesions of hepatocellular carcinoma and the regulation mechanism of the prescription mistletoe powder (HQS) and the monarch drug mistletoe on its activity.

　　Methods: Rats were divided into model group, HQS high-dose group [8 g/(kg·d)], low-dose group [4 g/(kg·d)], and mistletine group [8 mg/(kg·d)]. d)] and the normal group. The classic Solt-Farber two-step method was used to replicate the model of precancerous lesions in rats, and the expression of γ-glutamyl transpeptidase activity in liver tissue was detected by histochemical method; the expression of AFP in liver tissue was detected by immunofluorescence method; Quantitative Telomerase Detection Kit (QTD Kit) was used to determine the activity of telomerase in liver tissue; immunohistochemical method was used to detect the expression of NF-κB P65 protein in liver tissue; Western blot method was used to determine the content of IκB-α in cytoplasmic protein .

　　Results: After HQS and total mistletoe treatment, the area of γ-GT positive foci and the number of AFP positive cells in the liver were significantly reduced compared with those in the model group (P<0.05). Compared with the model group, the number of positive cells was significantly decreased (P<0.05); after treatment, the protein content of IκB-α in the cytoplasm was increased compared with the model group, and the difference was significant (P<0.05).

　　Conclusion: HQS and total alkaloids of mistletoe can inhibit the expression of telomerase activity in precancerous lesions of hepatocellular carcinoma, and their effect is to inhibit the overexpression of apoptosis-related gene NF-κB, increase the expression of IκB-α, and then reduce the expression of telomerase. active.