动物造模 z-bio 2022-07-21 371

　　Objective: To study the effect of polydatin (PD) on liver miR-214 and liver function in ApoE-/- mice.

　　Methods: Male ApoE-/- mice were fed with high-fat diet to establish mouse AS model. ApoE-/- mice were randomly divided into 4 groups (n=10), namely model group (Model), simvastatin group (Simvastatin), PD low-dose group (PDL), PD high-dose group (PDH), and the other group. Ten C57BL/6J mice of the same age were set as the normal control group. After continuous administration for 12 weeks, blood was collected to detect blood glucose, blood lipids, aspartate aminotransferase (AST), alanine aminotransferase (ALT), liver T-SOD and MDA and other indicators. miR-214 levels were detected.

　　Results: Compared with the normal control group, blood glucose, serum TC, TG, LDL-C, ALT and AST, and MDA in liver of mice in model group were significantly increased (P<0.01). miR-214 was significantly decreased (P<0.01), liver slices showed that the cells were filled with lipid droplets of different sizes, and most of the liver cells showed steatosis; compared with the model group, the PD high-dose group could significantly reduce the fasting blood glucose, the Serum TC, TG, LDL-C, AST, ALT and MDA in liver (P<0.05), and could significantly increase serum HDL-C and liver miR-214 and T-SOD (P<0.05); Compared with the model group, the hepatic steatosis of the mice in the low-dose group was alleviated.

　　Conclusion: PD can reduce blood sugar, blood lipids, and protect liver function in AS mice, and the mechanism may be mainly related to PD increasing the level of miR-214 in liver and regulating oxidative stress indicators such as T-SOD and MDA.