动物造模,诱发惊厥持续状态模型 z-bio 2022-07-21 591

　　OBJECTIVE: To investigate a method for reducing mortality in a lithium chloride-pilocarpine-induced status convulsive model by gastric gavage with modified oral rehydration salts.

　　Methods: 30 SD rats in the adult group (60 days) and 45 SD rats in the juvenile group (20 days) were randomly divided into adult SE+ORS group, adult SE group, juvenile SE+ORS group, juvenile SE group, There were 15 rats in the juvenile blood sugar group. Convulsive seizures were induced in rats by intraperitoneal injection of lithium chloride and pilocarpine, and 60 min after the convulsions, diazepam was given to stop the convulsions. Each ORS group was given different doses of modified ORS by gavage at 1, 12, and 24 h after SE. The other groups were not given treatment. The convulsions, body weight changes before and after convulsions, and death of the experimental rats were recorded; in the blood glucose measurement group, blood was collected at 8 h after SE to detect blood glucose, and to explore methods to reduce the mortality of the model.

　　Results: (1) The body weight of each group decreased after convulsion, accounting for about 5%-8% of the body weight. (2) The mortality rate of the lithium chloride-pilocarpine status convulsion model was higher, 40.00% in the juvenile group and 57.14% in the adult group. (3) After SE, the blood glucose of the experimental rats was significantly decreased, and the blood glucose tended to normal level after the ORS solution was administered by gavage. (4) After gavage with modified ORS, the mortality within 72 hours in the juvenile group and the adult group was significantly reduced.

　　Conclusion: The modified ORS solution gavage after SE can effectively improve the mortality of experimental animals, which is an effective method to improve the survival rate.