动物造模 z-bio 2022-07-21 373

　　Objective: To investigate the effect and molecular mechanism of solanine on the growth of prostate cancer cell Du145 transplanted in nude mice.

　　Methods: The highly malignant metastatic prostate cancer cell line Du145 was used as the model for animal in vivo experiments. Nude mice were subcutaneously inoculated with Du145 cells to establish a nude mouse subcutaneous tumor model. After 1 week, the inoculated nude mice were randomly divided into 2 groups: the solanine experimental group and the normal saline blank control group, and 0.2 mL of solanine (50 μg/mL) and normal saline were injected into the middle part of the solid tumor every 3 days, respectively. , observe the tumor growth in nude mice, 3 weeks later, the nude mice were killed by cervical dislocation, the tumor tissue was removed, the tumor weight was measured and the tumor inhibition rate was calculated according to the tumor weight. Real-time quantitative PCR and Western blotting techniques were used to detect the mRNA and protein expressions of tumor cell cycle-related genes in nude mice in each group. Tunel in situ detection of tumor tissue apoptosis in nude mice in each group.

　　Results: The tumor formation rate of nude mice in the solanine treatment group was significantly decreased, and the tumor growth rate of nude mice was significantly lower than that of the control group (P<0.01). Solanine can inhibit the expression of CyclinD1, CyclinE1, CDK2, CDK4 and CDK6 gene mRNA and protein in tumor cells, and significantly increase the expression of p21 gene mRNA and protein. After solanine intervention, tumor tissue apoptosis in nude mice was significantly increased ( P<0.01).

　　Conclusion: Solanine can inhibit the growth of prostate cancer cell xenografts in nude mice by regulating the G1/S checkpoint of the cell cycle and promoting tissue cell apoptosis.