小鼠实验,胰岛素抵抗 z-bo 2020-08-19 616

　　Many studies have shown that there is a certain correlation between protein hyperacetylation and impaired glucose tolerance and insulin resistance. This indicates that enzymes that regulate acetylation modification groups play an important role in the pathological process of sugar metabolism. Recently, American scientists published new scientific advances online in the International Journal of Diabetes. They found that the deacetylase sirt3 plays an important role in promoting glucose processing in the body, enhancing mitochondrial function and improving diet-induced insulin resistance.

　　Sirt3 is a mitochondrial NAD + dependent deacetylase. Previous studies have shown that Sirt3 plays an important role in regulating energy balance. Therefore, in this study, the researchers put forward an important hypothesis that the reduction of mitochondrial protein acetylation levels caused by the deletion of the sirt3 gene may promote adverse reactions caused by a high-fat diet. The researchers first discovered that using the hyperinsulin-positive glucose clamp experiment, sirt3-deficient mice had increased insulin resistance due to impaired glucose uptake in skeletal muscle.

　　Researchers then used the muscle fibers of sirt3 knockout mice on a high-fat diet to study muscle fibers and found that the tricarboxylic acid cycle was based on decreased substrate respiration and fatty acid-based respiration increased. This indicates that cell production has changed from using glucose as an energy source. Use fatty acids as energy conversion. This indicates that the skeletal muscle glucose uptake of sirt3 knockout mice fed a high-fat diet is reduced, and mitochondrial-bound hexokinase II (HKII) is also reduced, indicating that HKII activity is reduced. These results indicate that in a mouse model induced by a high-fat diet, lack of sirt3 can lead to a decrease in insulin-stimulated skeletal muscle glucose uptake and increase the dependence of skeletal muscle cells on fatty acids. However, sirt3 has been shown to have a depleting effect In rats, it will increase.

　　Does not damage the action of insulin. This study shows that skeletal muscle deacetylase sirt3 plays an important role in the response of skeletal muscle to insulin and the improvement of insulin resistance induced by high-fat diet.