动物造模 z-bio 2022-07-21 445

　　Objective: To investigate the expression and clinical significance of basic leucine zipper transcription factor ATF-like protein in kidney tissue of lupus mice.

　　Methods: 24-week-old female MRL/lpr mice were used as the lupus nephritis model group, and C57BL/6 female mice of the same age were used as the normal control group. Western blot was used to detect the protein expression of BATF in kidney tissue, and RT-PCR was used to detect the mRNA expression levels of BATF, RORγt and IL-17 in kidney tissue. , and analyzed the correlation of BATFmRNA, RORγt and IL-17 mRNA.

　　RESULTS: The levels of urine protein and serum anti-ds-DNA antibody in lupus mice were higher than those in normal mice (P<0.05). The histopathology of the kidneys of lupus mice mainly showed the proliferation of glomerular mesangial cells and the infiltration of a large number of inflammatory cells in the renal interstitium, while there was no obvious abnormality in normal mice. The expression levels of BATF mRNA and protein in kidney tissue of lupus mice were higher than those of normal mice (P<0.05). The expression of RORγt and IL-17 mRNA in the kidney tissue of lupus mice increased, and the expression of BATF mRNA was positively correlated with the expression of RORγt and IL-17 mRNA (r=0.945, 0.876, P<0.05).

　　Conclusion: The expression of BATF mRNA and protein in the kidney tissue of lupus mice is higher than that of the control group. BATF may participate in the pathogenesis of lupus nephritis by up-regulating Thl7 immune response.