动物造模,心肌纤维化 z-bio 2022-07-25 300

　　Objective: To investigate the effect of edaravone on reducing oxidative stress and anti-myocardial fibrosis.

　　Methods: Fifty 8-week-old SD male rats were randomly divided into 5 groups (control group, model group, and edaravone low, medium and high concentration groups). The rat myocardial fibrosis model was established by isoproterenol (ISO), and edaravone (Eda) dose groups were simultaneously intervened with edaravone for 14 days. On the 15th day, the degree of myocardial fibrosis, left ventricular mass index (LVMI), collagen volume fraction (CVF), and myocardial tissue type I, type III collagen (Col I, Col III) and hydroxyproline (Hyp) were detected in each group. , superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) contents, and the expression of TGF-β1 in myocardial tissue was detected by immunofluorescence and Western blot.

　　RESULTS: The CVF and LVMI of the model group were significantly higher than those of the control group (P<0.000), and the CVF and LVMI of the edaravone group showed a decreasing trend with the increase of the therapeutic concentration (low, medium and high concentration) (P<0.05). ); Col I, Col III and Hyp in the model group were significantly higher than those in the control group (all P 0.000), and with the increase of the treatment concentration, Col I, Col III and Hyp showed a downward trend; MDA in the model group was significantly higher than that in the control group (P =0.000), the levels of SOD and NO were significantly lower than those in the control group (all P0.000); SOD and NO in the low, medium and high concentration groups of edaravone were significantly higher than those in the model group (P<0.05); the model group TGF- β1 was significantly higher than that in the control group (P=0.000), and TGF-β1 in the low, medium and high concentration groups of edaravone was significantly lower than that in the model group; MDA was positively correlated with LVMI, CVF, Col I, Col III and Hyp, while SOD and NO was negatively correlated with LVMI, CVF, ColⅠ, ColⅢ, Hyp; TGF-β1 was positively correlated with LVMI, CVF, ColⅠ, ColⅢ, Hyp, MDA, but negatively correlated with SOD, NO.

　　Conclusion: Edaravone can reduce oxidative stress and inhibit the expression of TGF-β1 to delay myocardial fibrosis.