动物造模 z-bio 2023-05-25 516

　　Objective: To observe the induction effect of temozolomide on the programmed cell death of rat glioma C6 cells, and to explore the possible molecular mechanism.

　　METHODS: Different concentrations of temozolomide were used to treat rat brain glioma rat brain glioma C6 cell line at different time points, the inhibition rate was observed by MTT method, and the optimal effect time and concentration were screened out. The cells were treated with 400 μg/ml temozolomide for 24 h, and the induction effect of temozolomide on the apoptosis of rat brain glioma C6 cell line was observed by HE staining, Hochest, Western blot, Tunnel and immunohistochemistry.

　　Results: MTT assay, HE staining, Hochest and Tunnel results showed that temozolomide had apoptosis-inducing effect on rat glioma C6 cells; immunohistochemistry and Western blot results showed that the expression level of the pro-apoptotic protein Bax was up-regulated, and the apoptosis inhibitory protein Downregulation of Bcl-2 expression. No changes in expression of major caspase proteins were found.

　　Conclusion: Temozolomide has an apoptosis-inducing effect on glioma C6 cells, and this effect may be completed through a caspase-independent pathway.