动物造模 z-bio 2022-07-25 313

　　Objective: To explore the role of up-regulated expression of KA1 sub-receptors in the death of hippocampal neurons induced by endoplasmic reticulum stress.

　　Methods: Seventy adult male Kunming mice were randomly divided into kainic acid group (KA group), tunicamycin group (TM) 500 μg/ml group, TM1000 μg/ml group and TM2000 μg/ml group. KA or different concentrations of TM were injected, and brains were harvested by perfusion at different time periods (1, 2, 3, 4, 5, 8, and 12 h).

　　Results: At the 3rd, 4th, 5th, and 8th hours in the KA group and at the 4th and 5th hours in the TM2000 μg/ml group, Bederson's sign score showed significant damage to the central nervous system, FJB staining showed increased cell death in the hippocampus, and immunohistochemistry showed that KA1 and The expression of P-eIF2α in hippocampal neurons was significantly up-regulated (P<0.05).

　　Conclusion: The results of intrahippocampal injection of KA or TM show that KA1 is expressed in endoplasmic reticulum stress. In the process of neuronal apoptosis, the extramembrane receptor KA1 may first receive apoptotic signals and conduct them into cells, causing endoplasmic reticulum stress. Reticulum dysfunction, induce endoplasmic reticulum stress, and further promote neuronal apoptosis.