动物造模,肾缺血再灌注 z-bio 2022-07-25 340

　　OBJECTIVE: To investigate the effect of repetitive hypoxic preconditioning (RHP) on liver injury induced by renal ischemia-reperfusion injury (IRI) in rats and to preliminarily explore its mechanism.

　　METHODS: 120 male SD rats were randomly divided into 4 groups (n=30): hypoxia preconditioning surgery group (RHP group), hypoxia preconditioning sham surgery group (RHPS group), and normobaric surgery group (IRI group). and normal pressure sham operation group (S group). The rats were pretreated in a hypobaric oxygen chamber for 5 days to establish the renal IRI model. The RHP group and the IRI group were uniformly removed the right kidney, and the left renal hilum was clipped for 45 minutes, then released to establish the renal ischemia-reperfusion model. Only the right kidney was removed, and left kidney ischemia-reperfusion treatment was not performed. Serum alanine aminotransferase (ALT), IL-17A, and TNF-α concentrations were detected at 2, 8, and 24 h after reperfusion, and liver homogenate superoxide dismutase (SOD) and nitric oxide (NO) were detected by microplate reader. The levels of p-PI3K and p-AKT in the liver were detected by Western blot, and the structural changes of the liver were observed by histopathological examination.

　　RESULTS: Compared with the IRI group, the pathological and histomorphological examination of the rats in the RHP group at 2, 8, and 24 h after reperfusion showed that the injury was alleviated, the serum ALT concentration was decreased, and the TNF-α level was decreased at 24 h after reperfusion (P<0.05). ) NO content in liver tissue increased (P<0.05), SOD content increased at 8 h after reperfusion (P<0.05); compared with S group, serum IL-17A concentration in IRI group and RHP group were significantly increased (P<0.05). P<0.05), but="" there="" was="" no="" significant="" difference="" between="" the="" two="" groups="" p="">0.05). The expressions of P-PI3K and P-AKT in the RHP group were higher than those in the IRI group (P<0.05), and the difference was especially significant at 8 h after reperfusion. Significant (P<0.05).

　　Conclusion: RHP has a protective effect on the liver injury induced by IRI in the rat kidney, but it is not achieved by inhibiting IL-17A.