动物造模,肺缺血再灌注损伤 z-bio 2022-07-25 214

　　Objective: To investigate the effect and mechanism of emulsified fluorocarbon combined with ligustrazine on pulmonary ischemia-reperfusion injury.

　　Methods: Rat models of pulmonary ischemia-reperfusion were established and randomly divided into control group (C group), ligustrazine group (T group), emulsified fluorocarbon group (P group) and emulsified fluorocarbon combined with ligustrazine group (TP group). , 10 animals in each group were administered by tail vein 5 minutes before blood supply was restored, and animals were sacrificed 3 hours after blood supply was restored. Lung tissue was taken to measure malondialdehyde (MDA), myeloperoxidase (MPO) , superoxide dismutase (SOD), tumor necrosis factor-α (TNF-α) content, observe and score the pathological changes of lung tissue.

　　RESULTS: The contents of MDA and MPO in lung tissue of T group, P group and TP group were significantly lower than those of C group (P<0.05), and the MPO content of TP group was significantly lower than that of T group and P group (P<0.05). , TP group lung tissue SOD content was significantly higher than C group (P<0.05), TP group SOD activity was significantly higher than T group and P group (P<0.05); there was no significant difference in tnf-α content in the lung tissue of the four groups (P<0.05).>0.05); obvious edema was seen in the lung tissue of group C, red blood cells and exudation were seen in the alveolar space; no obvious edema was found in the lung tissue of T group and P group, but a small amount of red blood cells and exudation were seen; there was no obvious edema in the lung tissue of TP group, but a small amount of lung tissue was seen Exudation; the pathological score of group C was greater than that of group T, group P and TP, and the pathological score of group T and group P was greater than that of group TP (P<0.05).

　　Conclusion: Emulsified fluorocarbon and ligustrazine can improve the endogenous ability of scavenging oxygen free radicals, inhibit the accumulation of neutrophils in the lung, and reduce pulmonary ischemia-reperfusion injury, and the combined use of the two is more effective.